What forms of nutrients can Karenia brevis use to grow and bloom?

HAB researchers use laboratory experiments to test the types of nutrients that can be used by Karenia brevis, the Florida red tide organism. Results from this work may help managers develop strategies for mitigating blooms.

We need nutrients and so do algae. The major nutrients that algae use for growth are nitrogen (N) and phosphorus (P). They also use minerals and vitamins in smaller amounts.  Understanding the types of nutrients which harmful algae use to grow can help develop strategies for mitigating blooms. Florida Fish and Wildlife Conservation Commission (FWC) researcher, Dr. Bill Richardson, recently completed experiments designed to test the types of phosphorus that could be used by the red tide organism, Karenia brevis

Algae get P from dissolved compounds in the water. There are many different kinds of P-containing compounds dissolved in the sea, largely as the result of the natural decomposition of organisms, as well as from fertilizers and sewage. Among the explanations considered for the large and long-lasting blooms of the Florida red tide organism in the Gulf of Mexico include its ability to use a diversity of P-containing compounds and maybe even some compounds that other algae cannot use.

Test tubes  
K. brevis growing in experimental test tubes provided with different types of P-containing compounds.”  

Before this research, K. brevis was already known to use some P-containing compounds in the presence of bacteria, but its ability to use those compounds in the absence of bacteria had not been determined. In addition, bacteria in seawater are known to break down or mineralize P-containing compounds into smaller compounds more easily used by algae. To examine P use by K. brevis and the potential role of bacteria, laboratory cultures of K. brevis with bacteria and without bacteria were given a broad assortment of different P-containing compounds (29 in total) commonly found in seawater, and their growth on each different compound was followed. Scientists found the following results from this study:

  • K. brevis grew on many of the P-containing compounds in the absence of bacteria, indicating that K. brevis was able to directly use these compounds.

  • K. brevisdid not grow on some of P-containing compounds in the absence of bacteria but grew well on those compounds when bacteria were present, indicating that they were unable to directly use these compounds and that they depended upon the bacteria to break these compounds down into more readily usable forms.
Flourometer
Growth of K. brevis on each compound was followed using a fluorometer. Each day test tubes containing K. brevis were inserted into a fluorometer. The daily fluorometer readings were then used to calculate growth rates on the different compounds.”

 

These results suggest that large blooms of Karenia on the west Florida shelf are not the result of exceptional growth or the exclusive use of any specific inorganic or organic P compound. Instead, Karenia grows successfully on a broad spectrum of naturally occurring P compounds, either by taking P up directly or by taking it up indirectly after bacteria recycle it.

 

 

 



FWC Facts:
Although the Florida red tide is a naturally occurring phenomenon, agricultural and urban runoff can prolong red tide blooms inshore.

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